Miltenyi, 130-117-337, Classical Monocyte Isolation Kit, human

Background information
Non-monocytes, such as T cells, NK cells, B cells, dendritic cells, and basophils are indirectly magnetically labeled using a cocktail of biotin-conjugated antibodies and Anti-Biotin MicroBeads. Isolation of highly pure unlabeled monocytes is achieved by depletion of the magnetically labeled cells.

Downstream applications
- Functional studies on monocytes, in which any effect due to antibody binding to target cells should be avoided.
- Studies on monocyte activation, differentiation, cytokine secretion etc.
- Studies on compound or antigen uptake (e.g. phagocytosis) and antigen presentation by monocytes.
- In vitro differentiation of blood monocytes into dendritic cells or macrophages.

Columns
MS, LS, autoMACS® Columns, or Multi-24 Column Blocks

Overview
The Classical Monocyte Isolation Kit provides fast and consistent isolation of untouched classical ( CD14++CD16–) monocytes from peripheral blood mononuclear cells (PBMCs) for further functional studies. Depletion of non-monocytes is optimal and provides a highly pure population of label-free classical monocytes. This product includes an optional thrombocytes removal reagent to further reduce platelet contamination.