Description
Background information
The CD1c (BDCA-1) antigen is specifically expressed on dendritic cells, which are CD11chigh CD123low and represent the major subset of myeloid dendritic cells in human blood. CD1c (BDCA-1)+ dendritic cells show a monocytoid morphology (fig. 1) and express myeloid markers, such as CD13 and CD33 as well as Fc receptors, such as CD32, CD64, and FcεRI. Furthermore, they were determined to be CD4+, Lin (CD3, CD16, CD19, CD20, CD56)–, CD2+, CD45RO+, CD141 (BDCA-3)low, CD303 (BDCA 2)–, and CD304 (BDCA-4/ Neuropilin-1)–. A minor proportion of CD1c (BDCA-1)+ myeloid dendritic cells expresses CD14 and CD11b. CD1c (BDCA-1) is also found on CD1a+ dendritic cells generated ex vivo from monocytes or hematopoietic precursor cells, and on CD1a+ Langerhans cells in skin. Recently, a functionally different population of CD14+CD1c+ myeloid cells has been reported. To avoid the co-enrichment of such population, an optional removal reagent for CD14+ myeloid cells is included. In blood, apart from myeloid dendritic cells, a subset of small resting B cells expresses CD1c (BDCA-1). For this reason, the CD1c (BDCA-1)+ Dendritic Cell Isolation Kit includes CD19 MicroBeads for depletion of B cells prior to the enrichment of CD1c (BDCA-1)+ myeloid dendritic cells. In order to discriminate the CD1c (BDCA-1)+ from CD141 (BDCA-3)+ myeloid dendritic cells, they have been designated type 1 myeloid dendritic cells (MDC1s) or conventional DC2 cells (cDC2s).
Downstream applications
CD1c (BDCA-1)+ dendritic cells can be used for a variety of applications, for example:
- Isolation of CD1c (BDCA-1)+ myeloid dendritic cells to examine expression of Toll-like receptors, chemokine receptors, or new antigens, e.g., DCAL-1 and EMR2.
- Isolation for studies on dendritic cell activation, migration, cytokine production and T cell polarization, particularly in comparison with monocyte-derived dendritic cells.
- Isolation of CD1c+ DCs for studies evaluating new checkpoint inhibitors or dissecting their mechanisms of actions in tumor settings.
Columns
For the first magnetic separation (depletion): LD or autoMACS® Columns. For the second magnetic separation (positive selection): MS, LS, or autoMACS Columns.
Overview
The CD1c (BDCA-1)+ Dendritic Cell Isolation Kit has been developed for the isolation of CD1c (BDCA-1)+ myeloid dendritic cells (MDCs; type-1 MDC or cDC2) from PBMCs. This new format allows for the optional depletion of CD14+ myeloid cells that recently have been shown to possibly contaminate the final DC population.
