Background information
8-Color Immunophenotyping Cocktail, anti-human, REAfinity™ containing:
- CD3 Antibody, anti-human, PE, REAfinity (clone: REA613)
- CD4 Antibody, anti-human, Vio Bright 667, REAfinity (clone: REA623)
- CD8 Antibody, anti-human, APC-Vio 770, REAfinity (clone: REA734)
- CD14 Antibody, anti-human, VioBlue, REAfinity (clone: REA599)
- CD16 Antibody, anti-human, Vio Bright 515, REAfinity (clone: REA423)
- CD19 Antibody, anti-human, PE-Vio 770, REAfinity (clone: REA675)
- CD45 Antibody, anti-human, VioGreen™, REAfinity (clone: REA747)
- CD56 Antibody, anti-human, Vio Bright 515, REAfinity (clone: REA196)
CD4 Antibody, anti-human, Vio Bright 667, REAfinity (clone: REA623)
CD8 Antibody, anti-human, APC-Vio 770, REAfinity (clone: REA734)
CD19 Antibody, anti-human, PE-Vio 770, REAfinity (clone: REA675)
CD56 Antibody, anti-human, Vio Bright 515, REAfinity (clone: REA196)
Additionally
- 7-AAD Staining Solution
- Red Blood Cell Lysis Solution (10×)
Instrument details
For flow cytometric analysis use a flow cytometer equipped with a red (638 nm), a blue (488 nm), and a violet (405 nm) laser.
Applications
Evaluation of leukocyte subsets in whole blood, PBMCs, lysed whole blood samples, or other single-cell suspensions from human tissue.
Overview
The 8-Color Immunophenotyping Kit, anti-human, REAfinity is an advanced TBNK reagent to determine frequencies of immune cell populations (T, B, NK cells, monocytes, neutrophils, eosinophils, CD4+, CD8+, and CD56+CD3+ T cell subsets) in whole blood, PBMCs, or other human samples by flow cytometry. The kit includes a ready-to-use phenotyping cocktail, single antibodies for compensation and Red Blood Cell Lysis Solution. To ensure superior lot-to-lot consistency and low background, the kit applies recombinantly engineered REAfinity antibodies. The kit enables automated analysis for use with MACSQuant® Analyzer flow cytometers.
