Miltenyi, 130-120-673, MACSprep™ Chimerism CD34 MicroBead Kit, human

Background information
The isolation of CD34+ cells is performed with one labeling step without washing afterwards and in a two-step separation procedure. During the first isolation step red blood cells are aggregated and sedimented. In a second step, CD34+ cells are magnetically labeled with MACSprep Chimerism CD34 MicroBeads. Then, the cell suspension is loaded onto a MACS® Column, which is placed in the magnetic field of a MACS Separator. The magnetically labeled CD34+ cells are retained within the column. The unlabeled cells run through; this cell fraction is thus depleted of CD34+ cells. After removing the column from the magnetic field, the magnetically retained CD34+ cells can be eluted as the positively selected cell fraction.

Overview
MACSprep™ Chimerism CD34 MicroBead Kit has been developed for the fast isolation of CD34+ cells from freshly drawn anticoagulated whole blood without density gradient centrifugation nor red blood cell lysis.