Miltenyi, 130-121-273, CD90.1 MicroBeads, mouse and rat

Background information
The mouse monoclonal antibody reacts with rat CD90 (Thy-1) and mouse CD90.1 (Thy1.1), a GPI-anchored conserved membrane glycoprotein. In the rat, the CD90 antigen is expressed on thymocytes1, recent thymic emigrants1, hematopoietic stem cells, neurons, and other cell types. In the mouse strains AKR/J, PL, and FVB/N, CD90.1 is a pan T cell marker and can be found on thymocytes, hematopoietic stem cells in the bone marrow, intraepithelial cells (dendritic epidermal cells) in skin, and on neurons, such as retinal ganglion cells. The antibody does not cross-react with CD90.2 (Thy1.2).

Downstream applications
- CD90.1 MicroBeads are suitable for positive selection or depletion of mouse (strains AKR/J, PL, and FVB/N) or rat T lymphocytes from single-cell suspensions of spleen preparations or peripheral blood.
- Isolation of T cells for the adoptive transfer of CD90.1+ T cells into CD90.2+ inbred strains or immunodeficient mice.

Columns
For positive selection: MS, LS, or autoMACS® Columns. For depletion: LD or autoMACS Columns.

Overview
CD90.1 MicroBeads, mouse and rat, were developed for positive selection or depletion of mouse or rat T cells from single-cell suspensions of lymphoid and non-lymphoid tissues or from peripheral blood.