Miltenyi, 130-121-301, CD19 MicroBeads, mouse

Background information
The mouse monoclonal antibody reacts with mouse CD19, which is expressed on B cell lineage cells, throughout their development from early pro-B cell stages on, and is down-regulated upon terminal differentiation to plasma cells. Mouse CD19 MicroBeads are used for positive selection or depletion of mouse B cells from various tissues such spleen.

Downstream applications
- Positive selection or depletion of CD19+ B cells spleen.
- Isolation of CD19+ B cells to study the accessibility of enhancer elements during B cell development by in vivo footprinting.
- Isolation of pure B cells for preparation of cell extracts to analyze conditions for V(D)J recombination in a cell-free system.

Columns
For positive selection: LS or autoMACS® Columns. For depletion: LD or autoMACS Columns.

Overview
CD19 MicroBeads, mouse, were developed for positive selection or depletion of mouse B cells from single-cell suspensions of spleen.