Miltenyi, 130-122-357, Human IL-8, premium grade

Biological activity

Background information
Interleukin 8 (IL-8), also known as CXCL8, is a pro-inflammatory molecule belonging to the CXC chemokine family. The precursor form of IL-8 is cleaved into several mature isoforms (including 72 aa and 77 aa) and is secreted by several type of cells, especially monocytes and endothelial cells, upon pro-inflammatory stimulation. IL-8 signals through the CXCR1 and CXCR2 receptors, chemoattracts and activates granulocytes, predominantly neutrophils, T cells, and plays also an angiogenic role. Overexpression of IL-8 can lead to epithelial damage and injury of the airway tissues.

Quality description
Research-grade cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific activities are stated in the Certificate of Analysis (www. miltenyibiotec.com/certificates).

Overview
IL-8 stands for interleukin 8. Human IL-8 is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.