Miltenyi, 130-123-082, Calponin Antibody, anti-human, PE, REAdye_lease™

Name: Calponin Antibody, anti-human, PE, REAdye_lease™
Brand: Miltenyi
SKU: 130-123-082

Size:200 µL, Immunofluorescence analysis of a formalin-fixed, paraffin embedded (FFPE) human smooth muscle. Before staining, antigens were retrieved using heat-induced epitope retrieval (HIER) at pH 9. The section was stained using Calponin Antibody, anti-human, PE (green) for 10 min at room temperature (A). Calponin Antibody, anti-human, PE staining was released using the REAlease Release Reagent (B). Nuclei were visualized with Hoechst™ (blue). Scale bar: 30 µm.

Catalog Number: 130-123-082

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Category

Recombinant Antibodies

Pack Size200 µL

TagsMACS Antibodies Releasable fluorochromes

Description

Overview
Clone REAL635 is an antibody fragment derived from the full Calponin antibody molecule. It displays no binding to Fc receptors. The recombinantly engineered antibody fragments are multimerized to form the REAdye_lease Complex to bind markers with high avidity.
Clone REAL635 recognizes the human Calponin, a filament-associated calcium binding protein also known as Calponin-1 or CNN1. Calponin is strongly expressed in osteoblasts and smooth muscle cells. It binds several actin binding proteins and phospholipids e.g. actin, calmodulin, troponin C and tropomyosin. Calponin plays a role in the actin-myosin interaction and inhibits the ATPase activity of myosin in smooth muscle tissue.
Calponin-1 belongs to cancer-releated genes and operates as a biomarker particulary as prognostic marker in tumor cells.
For removal of REAdye_lease fluorochromes for optional relabeling with different fluorochrome-conjugated REAdye_lease antibodies use the REAlease Support Kit (130-120-675).

Specifications

General

Clone	REAL635
Clonality	monoclonal
Isotype control	Control Antibody
Host	cell line
Type of antibody	Releasable fluorochromes, Primary antibodies, Recombinant antibodies
Species reactivity	human
Cross-reactivity	rat
Antigen	Calponin
Distribution of antigen	osteoblasts, tumor cells, other, smooth muscle
RRID	AB_2857573

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