Description
Background information
Stem cell antigen-1 (Sca-1) is one of the defining markers for KSL cells (c-kit+ Sca-1 Lin–), the population that comprises the stem cell compartment in mouse bone marrow. Sca-1 expression in combination with CD105 can be further used to define long-term repopulating hematopoietic stem cells (LTR-HSCs).6,7
The Sca-1 protein is encoded by one of the two Ly-6A/E alleles expressed in different mouse strains (Ly-6E.1: e.g. BALB/c, C3H, NZB; Ly-6A.2: e.g. C57BL/6, SJL, 129, AKR). The anti-Sca-1 monoclonal antibody included in the kit recognizes both, Ly-6E.1 and Ly-6A.2.
Downstream applications
Bone marrow-derived Sca-1+ cells have been used for nonhematopoietic differentiation into hepatocytes in vivo8 and neural cells in vitro9. ES cell-derived SCA-1+ cells could be differentiated into endothelial cells with high purity and yield.10 Furthermore, smooth muscle cells have been generated from ESCs via isolation of Sca-1+ cells.11
Columns
MS, LS, XS, or autoMACS® Columns.
Overview
The Anti-Sca-1 MicroBead Kit (Vio® Bright FITC), mouse has been developed for the positive selection of hematopoietic stem and progenitor cells from mouse bone marrow. Sca-1+ stem cells have been isolated from peripheral blood1, fetal2, and adult liver3, as well as heart4 and prostate tissue5. The kit can be used either alone or in combination with the Lineage Cell Depletion Kit, mouse for enrichment of KSL cells as well as in combination with the CD105 MultiSort Kit (PE), mouse for the enrichment of long-term repopulating hematopoietic stem cells (LT-HSCs).
