Description
Background information
The Dengue virus can be found in tropical and subtropical areas worldwide and belongs to the family of flaviviruses. Dengue fever is a mosquito transmitted viral infection and can be caused by four distinct serotypes (DEN-1, DEN-2, DEN-3, and DEN-4), inducing high fever, severe muscle pain, and nausea. Dengue virus type 2 (DENV-2) is the most common infecting serotype. Recovery from infection by one Dengue virus induces immunity against the homologous serotype but not against the other three serotypes. Secondary infection with a different DENV serotype can cause the potentially life-threatening dengue haemorrhagic fever (DHF) and dengue shock syndrome (DSS).
Downstream applications
PepTivator® Dengue Virus Type 2 Glycoprotein M – research grade has been specifically developed for efficient in vitro stimulation of Dengue–specific T cells. Peptides of 15 amino acids in length and 11 amino acids overlap represent an optimized solution for stimulating both CD4+ and CD8+ T cells in various applications, including:
- Detection and analysis of antigen-specific CD4+ and CD8+ effector/memory T cells in PBMCs by MACS® Cytokine Secretion Assays, intracellular cytokine staining, or other technologies.
- Isolation of viable antigen-specific CD4+ T cells with the CD154 MicroBead Kit, or of CD4+ and CD8+ T cells using the CD137 MicroBead Kit or MACS Cytokine Secretion Assay – Cell Enrichment and Detection Kits. Subsequently, cells can be expanded for generation of T cell lines.
- Generation of antigen-specific effector/memory T cells from naive T cell populations.
- Pulsing of antigen-presenting cells.
Overview
The PepTivator® Dengue Virus Type 2 Glycoprotein M is a pool of lyophilized peptides, consisting mainly of 15-mer sequences with 11 amino acids overlap, covering the sequence of the Dengue Virus Type 2 Glycoprotein M. The sequence is derived from UniProt Acc. no. P14340 (POLG_DEN2N) and covers aa 115–284 of the genome polyprotein.
In vitro stimulation of antigen-specific T cells with PepTivator Peptide Pools causes the secretion of effector cytokines and the up-regulation of activation markers, which then allow the detection and isolation of antigen-specific T cells.
