Description
Background information
CD163 is most abundantly expressed by mature tissue macrophages and peripheral blood monocytes. CD163 expression is up-regulated in vitro and in vivo by anti-inflammatory mediators.Depending on the ligand, crosslinking of CD163 initiates signal transduction leading to the production of pro-inflammatory cytokines, such as IL-1ß, IL-6, and GM-CSF or the anti-inflammatory cytokine IL-10.
Detailed separation procedure
The CD163+ cells are indirectly magnetically labeled with CD163-Biotin and Anti-Biotin MicroBeads. The magnetically labeled CD163+ cells are retained in a MACS® Column in the magnetic field of a MACS Separator, while the unlabeled cells pass through the column. The CD163+ cells can then be eluted by removing the column from the magentic field.
Downstream applications
- Positive selection of human cells expressing the CD163 antigen
- Isolation or depletion of CD163+ cells from human peripheral mononuclear cells or single-cell suspensions from, e.g. tumor tissues
- Enrichment of CD163+ macrophages from single-cells suspension of human tumor samples
Columns
MS, LS, or autoMACS® Columns.
Overview
The CD163 MicroBead Kit, human has been developed for the enrichment of human cells based on the expression of the CD163 antigen. CD163 is most abundantly expressed by mature tissue macrophages and peripheral blood monocytes. The expression of CD163 is up-regulated in vitro and in vivo by anti-inflammatory mediators. Depending on the ligand, crosslinking of CD163 initiates signal transduction leading to the production of proinflammatory cytokines, such as IL-1ß, IL-6, and GM-CSF or the anti-inflammatory cytokine IL-10.
