Miltenyi, 130-124-787, Mo-DC Generation Toolbox, human

Downstream applications
For the isolation of monocytes from PBMCs and subsequent differentiation of immature and mature monocyte-derived dendritic cells in vitro. Common uses for Mo-DCs are cellular vaccine development, antigen-specific T cell stimulation, as well as toxicity and hypersensitivity testing.

Columns
MS, LS, or autoMACS® Columns.

Overview
The Mo-DC Generation Toolbox comprises reagents for magnetic cell separation of CD14+ monocytes and the subsequent differentiation and maturation to monocyte-derived dendritic cells (DCs). Large numbers of Mo-DCs can be generated ex vivo from purified monocytes (easily isolated from bone marrow, cord blood, peripheral blood, or peripheral blood mononuclear cells (PBMCs)). The excellent purity and recovery of selected monocytes offer a standardized and consistent cell source. The Mo-DC Differentiation Medium contains interleukin-4/GM-CSF. Mo-DCs can easily be matured with the premium grade TNF-α provided with the kit. The lot-to-lot consistency and standardized protocol we offer enable for effective differentiation of immature and mature Mo-DCs.