Miltenyi, 130-125-042, StemMACS™ HSC-CFU Assay Kit, human

Background information
The StemMACS HSC-CFU Assay Kit offers a highly standardized method for analyzing hematopoietic stem and progenitor cells. The kit combines differentiation in methylcellulose-free cell culture with a standardized, flow cytometry-based read-out and eliminates the need for user-dependent, visual scoring under a microscope.

For the colony identification via flow cytometric readout when using the MACSQuant Analyzer, we provide an analysis excel sheet and a gating template. These analysis tools are optimized for the MACSQuantify Software version 2.11 and upwards. To receive these analysis tools, please contact your local technical support.

Applications
- Characterization of CD34+ cells in their differentiation potential by CFU
- Option for the measurement of GFP-modified cells

Workflow using the StemMACS HSC-CFU Assay Kit. HSCs, diluted in StemMACS HSC-CFU Assay Media, are plated in 96-well plates. After proliferation and differentiation for 14 days, the generated colonies are stained with the StemMACS HSC-CFU Assay Cocktail and analyzed by flow cytometry. BFU-E: burst-forming unit-erythroid, CFU-G: colony-forming unit-granulocyte, CFU-M: CFU-macrophage, CFU-GM: CFU-granulocyte macrophage, CFU-GEMM: CFU-granulocyte erythrocyte macrophage megakaryocyte.

Overview
StemMACS™ HSC-CFU Assay Kit has been developed for the expansion of CD34+ cells and the evaluation of their differentiation potential as Colony Forming Units (CFU) using a standardized flow cytometry-based read-out.