Miltenyi, 130-125-289, StemMACS™ CardioDiff Kit XF, human

Background information
Directed differentiation of specific lineages from human pluripotent stem cells (hPSCs) is a major tool for developmental or disease models, drug screening platforms, and cellular therapies. For all these applications, it is pivotal to have a consistent, time-saving, and reliable differentiation method.
StemMACS CardioDiff Kit XF is a complete, ready-to-use, and xeno-free cell culture system for efficient and fast differentiation of hPSCs into cardiomyocytes. The kit is composed of three phenol red–free media that progressively restrict the cellular fate and promote the differentiation into cardiomyocytes in just 8 days of culture. Depending on the cellular line, first contracting cardiomyocytes can be observed already after 6 days of culture. Moreover, the protocol can be up-scaled without differences in the efficiency rates. PSCs-derived cardiomyocytes can be further expanded in StemMACS Cardiac Cultivation Medium and cultivated for more than 30 days.

Applications
- Directed differentiation of cardiomyocytes from hPSCs lines

Overview
StemMACS CardioDiff Kit XF is a complete ready-to-use, and xeno-free cell culture system for efficient and fast differentiation of human pluripotent stem cells (PSCs) into cardiomyocytes.