Description
Background information
The mouse CD11c antigen is present in dendritic cells in lymphoid organs and blood, in Langerhans cells in the epidermis, in dendritic cell progenitors in the bone marrow, and in in vitro generated bone marrow–derived dendritic cells. In mouse spleen and lymph node, CD11c is expressed at high levels on conventional CD11c+CD45R–mPDCA-1– dendritic cells, and at moderate levels on CD11c+CD45R+ mPDCA-1+ plasmacytoid dendritic cells. CD11c is reported to be weakly expressed on NK cells, B cells, and T cell subsets. About 1–3% of splenocytes, 2% of bone marrow cells, as well as <1% of lymph node cells and thymocytes express CD11c.
Downstream applications
DCs isolated with CD11c MicroBeads have been used for phenotypical and functional analyses, e.g. T cell activation, polarization, and tolerance induction in different experimental murine models. CD11c MicroBeads were used to isolate highly pure DCs from spleen and lymph nodes1,2, Peyer's patches3, colonic lamina propria4, bone marrow5, epidermis6, lung7, liver8, or brain9
Columns
For positive selection: MS, LS, XS, or autoMACS® Columns. For depletion: LD, D, or autoMACS Columns.
Overview
In mice CD11c is a well-established, yet not exclusive, pan-DC marker. CD11c MicroBeads UltraPure have been optimized for the isolation of mouse DCs from single-cell suspensions from lymphoid and non-lymphoid tissues. The CD11c MicroBeads UltraPure allow for the rapid and simple isolation of all defined murine DC subsets. The UltraPure beads greatly improve recovery and purity of the sorted population by specifically enriching viable cells.
