Description
Overview
Clone REAL864 is an antibody fragment derived from the full Bcl-2 antibody molecule. It displays no binding to Fc receptors. The recombinantly engineered antibody fragments are multimerized to form the REAdye_lease Complex to bind markers with high avidity.
Clone REAL864 recognizes the human Bcl-2 antigen, which is an intracellular single-pass membrane protein found primarily in the mitochondrion outer membrane, the nucleus membrane, and the endoplasmic reticulum membrane. Bcl-2 is a member of the Bcl-2 family of regulator proteins and forms homodimers or heterodimers with other Bcl-2 family members. It suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Bcl-2 regulates cell death by controlling the mitochondrial membrane permeability and it appears to function in a feedback loop system with caspases. Caspase activity is either inhibited by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor. BCL-2 antibody is suited for the differentiation between reactive and neoplastic follicular proliferation in lymph nodes and could be a useful marker for breast cancer and non-small cell carcinoma of the lung.
For removal of REAdye_lease fluorochromes for optional relabeling with different fluorochrome-conjugated REAdye_lease antibodies use the REAlease Support Kit (130-120-675).
