Miltenyi, 130-126-335, MACS® Imaging Solution

Background information
Working with transparent tissues is essential for fluorescence imaging of large biological systems in three dimensions (3D). However, most tissues are opaque and need to be treated with chemical components in order to render them transparent, a process also known as tissue clearing.
For 3D imaging analysis, the cleared tissues need to be embedded in a solution that matches their refractive index. The specific refractive index of a tissue after clearing is given by the refractive index of the reagent used to render the tissue transparent.
The MACS® Imaging Solution can be used for the 3D imaging analysis of tissues with a refractive index of 1.556–1.562, such as tissues that have been cleared using the MACS® Clearing Kit.

Overview
The MACS Imaging Solution is a non-toxic organic solvent with a refractive index of 1.556-1.562 that enables the three dimensional (3D) imaging analysis of cleared tissues using different microscopy systems, such as light sheet fluorescence microscopy, confocal or two-photon microscopy. Cleared tissues with a matching refractive index to the MACS Imaging Solution, are embedded in this solution in the imaging chamber of the microscopy system of choice for their downstream 3D imaging analysis.
Please note: For use with the UltraMicroscope Blaze, we recommend ordering the MACS Imaging Solution, 2x450 mL (130-128-511) providing a sufficient volume to fill the instrumtent´s image chamber (500 mL).