Description
Background information
SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2), also known as novel coronavirus 2019-nCoV, causes fever, severe respiratory illness and can lead to life threatening pneumonia. The first cases of this disease, termed COVID-19 for coronavirus disease 2019 have been detected in December 2019 in Wuhan, China.
SARS-CoV-2 Prot_M stands for the membrane glycoprotein of SARS-CoV-2. This protein is known to shape the viral envelope of coronaviruses and is involved in virion assembly as well as budding. Thus, the membrane glycoprotein (“M”) of SARS-CoV-2 has been suggested as possible target for vaccine development.
Applications
PepTivator® SARS-CoV-2 Prot_M – research grade has been specifically developed for in vitro stimulation of SARS-CoV-2–specific T cells. Peptides of 15 amino acids in length and 11 amino acids overlap represent an optimized solution for stimulating both CD4+ and CD8+ T cells in various applications, including:
- Detection and analysis of SARS-CoV-2–specific CD4+ and CD8+ effector/memory T cells in PBMCs by MACS® Cytokine Secretion Assays, intracellular cytokine staining, or other technologies.
- Isolation of viable SARS-CoV-2–specific CD4+ T cells with the CD154 MicroBead Kit, or of viable CD4+ and CD8+ T cells using MACS Cytokine Secretion Assay – Cell Enrichment and Detection Kits. Subsequently, cells can be expanded for generation of T cell lines.
- Generation of SARS-CoV-2–specific CD4+ and CD8+ effector/ memory T cells from naive T cell populations.
- Pulsing of antigen-presenting cells, e.g. for research on dendritic cell vaccination.
More information regarding the work with antigen-specific T cells can be found in the T cell application section.
Overview
Stimulate T cells reactive to the membrane glycoprotein of the SARS-CoV-2 Wuhan wild-type using PepTivator Peptide Pools. PepTivators are pools of lyophilized peptides, consisting mainly of 15-mer sequences with 11 amino acids overlap. PepTivator SARS-CoV-2 Prot_M is a pool of lyophilized peptides, covering the complete sequence of the membrane glycoprotein (“M”) of SARS-Coronavirus 2 (GenBank MN908947.3, Protein QHD43419.1).
In vitro stimulation of antigen-specific T cells with PepTivator Peptide Pools causes the secretion of effector cytokines and the up-regulation of activation markers, which then allow the detection and isolation of antigen-specific T cells.
