Description
Background information
Tissue clearing refers to the process of rendering opaque tissues optically transparent. Working with transparent tissues is required for the imaging analysis of large biological systems in three dimensions (3D).
The MACS® Clearing Kit provides reagents for the immunostaining and clearing of different tissues. Briefly, samples are first fixed and permeabilized prior immunostaining. The duration of the immunostaining step is dependent on the tissue. After immunostaining, tissues are dehydrated using a series of ethanol dilutions and afterwards treated to render them transparent (cleared). Cleared tissues can be analyzed using the MACS® Imaging Solution in different microscopy systems.
Specific protocols using the MACS® Clearing Kit are available for different tissues, such as mouse brain hemispheres, cerebral organoids, and human tumors. The MACS® Clearing Kit is supplied with sufficient amounts to clear high number of tissues, such as for instance up to 20 mouse brain hemispheres.
Duration of steps for clearing opaque tissues for 3D imaging using the MACS® Clearing Kit. Duration of immunostaining step is based on the use of directly conjugated antibodies.
Overview
The MACS® Clearing Kit enables the immunostaining and clearing of different tissues for their subsequent three dimensional (3D) imaging analysis. Specific protocols have been developed using the MACS® Clearing Kit for the easy, fast and effective immunostaining and clearing of different tissues, such as mouse brain hemispheres, cerebral organoids, and human tumors. After clearing, tissues can be analyzed using different microscopy systems, such as light sheet fluorescence microscopy, confocal or two-photon microscopy.
