Description
Background information
The updated “version 02” offers improved performance and an advanced and revised marker selection, containing predominantly our REAfinity™ Recombinant Antibodies. REAfinity Antibodies are generated in a biologically and chemically defined in vitro expression system to guarantee maximum purity and lot-to-lot consistency. The genetic sequence is engineered to lack binding to Fcγ receptors (FcγRs). Binding to FcγRs is one of the main causes of background signal in flow cytometry analysis. In addition, use of APC-conjugated antibodies in MACS Marker Screen, anti-human, version 02 allows for convenient screening of GFP- or YFP-expressing cells.
Predefined experiment tables containing well annotations and basic instrument settings for MACSQuant® Instruments allow for convenient annotation and analysis of samples.
4× MACS Marker Screen Plates, anti-human, version 02 (96-well round bottom plates) containing 384 pre-titrated anti-human antibodies, including 6 isotype controls, conjugated to APC.
Additionally
- 8× adhesive foils
Instrument details
Flow cytometer equipped with a red (640 nm) laser.
Applications
Characterization or profiling of cell lines or primary cells and cell mixtures. Identification of expression changes with different experimental conditions or development of cell separation strategies.
Overview
The MACS® Marker Screen, anti-human, version 02 has been designed for screening and analysis of 378 human surface markers by flow cytometry. The kit combines the benefits of flow cytometry and high quality pre-titrated antibodies to screen for markers of interest in a standardized and high throughput manner. Identified markers or combinations of markers can serve to better characterize cell compositions or cells of interests, e.g., for quality control purposes. Furthermore, markers can be utilized to develop target cell isolation strategies.
