Miltenyi, 130-127-439, PSC-mDA Neuron Analysis Kit, anti-human, REAfinity™

Name: PSC-mDA Neuron Analysis Kit, anti-human, REAfinity™
Brand: Miltenyi
SKU: 130-127-439

Size:for 30 tests

Catalog Number: 130-127-439

Pricing

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Category

Soil Test Kits

TagsMACS Flow Cytometry Flow cytometry reagents and kits Phenotyping assays

Description

Background information
PSC-mDA Neuron Analysis Kit, anti-human, REAfinity™
Oct3/4 Antibody, anti-human/mouse, APC, REAfinity (clone: REA622)
FoxA2 Antibody, anti-human, APC, REAfinity (clone: REA506)
OTX2 Antibody, anti-human, Vio® B515, REAfinity (clone: REA1178)
PAX-6 Antibody, anti-human, PE, REAfinity (clone: REA507)
TTF-1 Antibody, anti-human, Vio B515, REAfinity (clone: REA1090)
Sox1 Antibody, anti-human, PE, REAfinity (clone: REA698)

Additionally
- Fixation/Permeabilization Solution 1
- Fixation/Permeabilization Solution 2
- Permeabilization Buffer (10×)

Instrument details
Flow cytometer equipped with a red (640 nm) and a blue (488 nm) laser.

Applications
Flow cytometry-based quality control kit for in vitro phenotyping of identity and purity of PSC-derived mDA neurons during differentiation.

Overview
The PSC-mDA Neuron Analysis Kit, anti-human, REAfinity has been developed as a flow cytometry-based quality control assay to evaluate purity and identity of midbrain dopaminergic (mDA) neurons differentiated from PSCs. The kit takes advantage of the fast approach that flow analysis provides and combines it with the high staining specificity and low background of REAfinity Antibodies. The qualitative and quantitative analysis allows to determine the overall success of the differentiation protocol and define minimal criteria for the differentiation performances to ensure consistency between rounds of differentiations.

Ordering

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