Miltenyi, 130-127-497, HA Antibody, Biotin

Name: HA Antibody, Biotin
Brand: Miltenyi
SKU: 130-127-497

Size:30 tests in 60 µL, 293HEK cells transiently transfected with HA-tagged CD4 were stained intracellularly with Anti-HA-FITC and CD4-PE, and analyzed by flow cytometry to control gene-of-interest and MACSelect™ surface marker expression.

Catalog Number: 130-127-497

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Category

Biotin-Conjugated Secondary Antibodies

Pack Size30 tests

TagsMACS Antibodies Hybridomas

Description

Overview
The monoclonal Anti-HA antibody facilitates the fast and convenient detection of HA-tagged fusion proteins, expressed in either prokaryotic or eukaryotic cells. The antibody is available as a conjugate with horseradish peroxidase (HRP), fluorochromes, or biotin for fast and convenient analysis of HA-tagged fusion proteins. Conjugation of the Anti-HA antibody to HRP simplifies Western blot or ELISA analysis as the incubation with secondary antibodies becomes dispensable. Flurochrome-conjugated Anti-HA antibodies enable direct flow cytometry and fluorescence microscopy analyses, while indirect fluorescent labeling of HA fusion protein expressing cells is possible with the biotin-conjugated antibody. After incubation the Anti-HA-HRP antibody can be directly detected using commercially available chemiluminescent reagents.
The monoclonal Anti-HA antibody specifically recognizes proteins that are tagged with the HA epitope (YPYDVPDYA).

Specifications

General

Clone	GG8-1F3.3.1
Clonality	monoclonal
Isotype	mouse IgG1
Host	mouse
Type of antibody	Primary antibodies, Epitope tag antibodies
Species reactivity	any
Antigen	HA
Molecular mass of antigen [kDa]	1.1
Distribution of antigen	any cell type
RRID	AB_2921809, AB_2784358, AB_2784357, AB_871936, AB_2921810, AB_2784359, AB_2802052, AB_2802046, AB_2784360

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