Miltenyi, 130-127-857, Human FGF-10, premium grade

Biological activity

Background information
Fibroblast growth factor 10 (FGF-10) belongs to the FGF family, which is involved in embryogenesis and adult tissue development and regeneration. The function of FGF-10 partially overlaps with the one of other FGF members such as FGF-7. It is expressed in mesenchymal cells especially in lungs, and signals via FGFR 2. FGF-10 influences the development of several tissues including lungs and adipose tissues, and its activity is inhibited by SHH and BMP-4. Human FGF-10 shares 93% amino acid sequence with mouse FGF-10.

Quality description
Research-grade cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific activities are stated in the Certificate of Analysis (www. miltenyibiotec.com/certificates).

Overview
Recombinant human FGF-10 (Fibroblast growth factor 10) is used for differentiation of ES/iPS cells. The cytokine is critical during embryogenesis, e.g. FGF-10 is essential for the development of epithelial cells in the lung. Human FGF-10 recombinant protein is developed for use in cell culture, differentiation studies, and functional assays.