Description
Background information
StemMACS PSC-Brew XF, human is a new generation cell culture medium for highly efficient maintenance and expansion of human pluripotent stem cells (hPSC). It has a xeno-free and serum-free formulation that ensures a reduced variability and standardized performances during culture. The carefully balanced composition and the presence of stable FGF-2 ensures constant exposure levels and provides flexibility with feeding schedules.
StemMACS PSC-Brew XF, human is compatible with both defined and undefined cell attachment matrices and allows the maintenance of a highly consistent pluripotent phenotype even in low density cultures. hPSCs grown in StemMACS PSC-Brew XF, human show a typical morphology, expression of pluripotency-associated markers and retain the ability to differentiate into cell types that derive from the three embryonic germ layers. Moreover, the maintenance of a stable karyotype in long-term cultivation is supported.
StemMACS PSC-Brew XF, human allows rapid culture re-initiation of hPSC cultures after cryopreservation and enables cluster passaging without the addition of any inhibitor.
StemMACS PSC-Brew XF, human is fully compatible with StemMACS PSC-Support XF, human and, when combined, can be used in challenging applications that would normally stress the cells, such as gene editing approaches or after reprogramming.
Applications
Highly efficient maintenance and expansion of hPSCs under xeno- and feeder-free conditions.
Overview
StemMACS PSC-Brew XF, human is a new generation culturing medium for highly efficient maintenance and expansion of human pluripotent stem cells (hPSC).
