Description
Background information
The MACSprep CD19 CAR MicroBead Kit, human has been developed for the separation of cells that are engineered to express CD19-specific chimeric antigen receptor (CAR) on the cell surface.
The kit contains a cocktail and a buffer to remove most of RBCs. In this RBC-reduced blood sample the engineered CD19 CAR T cells can be detected via the CD19 CAR Detection Reagent, human, Biotin and can be enriched by magnetic labeling using Anti-Biotin MicroBeads.
Applications
Isolation of CD19 CAR cells from whole blood. The purified CD19 CAR cells are well suited for further flow cytometric, functional, or molecular analysis.
Overview
The MACSprep™ CD19 CAR MicroBead Kit, human has been developed for the fast isolation of CD19 CAR cells from freshly drawn anticoagulated whole blood without density gradient centrifugation nor red blood cell (RBC) lysis. During the first isolation step RBCs are aggregated and sedimented. In the second step, CD19 CAR cells are labeled with CD19 CAR Detection Reagent, human, Biotin. After one washing step the CD19 CAR Detection Reagent-bound cells are magnetically labeled with Anti-Biotin MicroBeads. Then, the cell suspension is separated by loading it onto a MACS® Column, which is placed in the magnetic field of a MACS Separator. The magnetically labeled CD19 CAR cells are retained within the column. The unlabeled cells run through, this cell fraction is thus depleted of CD19 CAR cells. After removing the column from the magnetic field, the magnetically retained CD19 CAR cells can be eluted as the positively selected cell fraction.
