Miltenyi, 130-128-024, Nuclei Extraction Buffer

Background information
The Nuclei Extraction Buffer allows for the preparation of single nuclei suspensions from a wide range of tissues types including brain, liver, heart, pancreas and kidney, as well as human and mouse solid tumors. Our method is optimized for the extraction of nuclei from snap-frozen tissue and it is also applicable for OCT embedded and fresh tissues.
The Nuclei Extraction Buffer is to be used with the gentleMACS Octo Dissociators and C Tubes and integrates tissue dissociation and cell lysis in a single step. You can extract nuclei in seven minutes at 4°C and run up to eight samples in parallel. Our standardized nuclei extraction method enables the generation of high quality nuclei suspensions to high yields.

Downstream applications
The nuclei extraction method is compatible with a wide variety of applications, including single nucleus gene expression analysis and single nucleus ATAC-seq. Some applications, e.g. single nucleus ATAC-seq, required nuclei permeabilization after extraction.
For details on the permeabilization, please follow the recommendation of the genomic application provider.

Overview
The Nuclei Extraction Buffer is designed to gently, rapidly, and effectively generate single nuclei suspensions from fresh and frozen tissues. It has been developed for use with the gentleMACS Dissociators and C Tubes.