Miltenyi, 130-128-760, MACS®qPCR LENTIcheck Kit (VSV-G), human

Overview
The MACS® qPCR LENTIcheck Kit (VSV-G), human is a qPCR-based assay developed for the rapid assessment of replication competent lentivirus (RCL) in cell-based products transduced with lentiviral vectors provided by Lentigen and Miltenyi Biotec. The MACS qPCR LENTIcheck Kit (VSV-G) detects the lentiviral envelope gene sequences (vesicular stomatitis virus G glycoprotein [VSVg]). VSVg is thought to be the most suitable target for detecting RCL as incorporation of the envelope gene sequence is required to generate a replication competent virus. The absence of the VSV-G gene in ex vivo transduced cells according to the PCR assay can be used for lot release testing of cell-based products, in particular, when time constrains are present.
Important note: The primers included in the MACS qPCR LENTIcheck Kit (VSV-G), human are designed to amplify an amplicon of the lentiviral envelope gene sequence (VSVg). These primers are intended to be used with the lentiviral vectors provided by Lentigen and Miltenyi Biotec. For information about compatibility with other lentiviral vectors, please contact the Miltenyi Biotec Technical Support team.