Description
Background information
The Multi-24 Whole Column Block in combination with StraightFrom® Whole Blood MicroBeads or StraightFrom® Buffy Coat MicroBeads or MACSprep™ Chimerism MicroBeads allows cell separation directly from whole blood or buffy coat without density-gradient centrifugation and erythrocyte lysis. Optimal flow rates of whole blood samples during cell separation are achieved by a specially developed column matrix.
The matrix of each column consists of ferromagnetic spheres. The matrix is covered with a cell-friendly coating. When placed in the magnetic field of a MultiMACS™ Cell24 Separator Plus or the MultiMACS™ X, the spheres amplify the magnetic field by 10,000-fold, thus inducing a high gradient within the column. This is crucial for isolation of cells that are only minimally labeled with MACS® MicroBeads, leaving enough epitopes free for concurrent antibody staining. The space between the spheres is several times larger than primary and most cultured cells. This allows the cells to freely flow through the column. Magnetically labeled cells are held in suspension within the column and do not actually “bind” the column matrix. This suspension minimizes stress on the cells and allows for efficient sterile washing by avoiding cell aggregation.
The Multi-24 Whole Column Block are sterile, single packed.
Please note: The Whole Blood Column Elution Buffer is not provided with the Multi-24 Whole Blood Column Block. It has to be purchased separately. One bottle of 50ml is sufficient to elute 12 columns, for the all block elution 2x50ml of Whole Blood Column Elution Buffer are needed (#130-131-889).
Overview
The Multi-24 Whole Blood Column Block is designed for isolation of cells directly from Whole Blood and Buffy Coat used in combination with the MultiMACS™ Cell24 Separator Plus and the MultiMACS X. The product enables the parallel separation of large sample volumes or up to 24 individual samples in one run by combining 24 Whole Blood Columns in one block. Please contact technical support for further information.
