Miltenyi, 130-132-045, PepTivator® M. tuberculosis ESAT-6, premium grade

Background information
Tuberculosis (TB) caused by infection with Mycobacterium tuberculosis is one of the leading causes of death worldwide. According to the WHO a quarter of the world’s population is infected with M. tuberculosis. While most infections are latent and asymptomatic, about 5–10% of infections progress to active disease. TB persists as a serious global health threat, requiring new vaccines and therapeutics to combat multidrug resistance. ESAT-6 (early secreted antigenic target 6 kDa) is an abundantly secreted protein of M. tuberculosis that has been identified as major virulence factor and potent inducer of antigen-specific T cell responses. ESAT-6 forms a 1:1 heterodimeric complex with CFP-10 (culture filtrate protein 10 kDa) that is secreted via the ESX-1 secretion system and attaches to the cell membrane of host macrophages and monocytes.

Overview
Stimulate T cells reactive to the ESAT-6 antigen of Mycobacterium tuberculosis using PepTivator® Peptide Pools. PepTivators are pools of lyophilized peptides, consisting mainly of 15-mer sequences with 11 amino acids overlap. PepTivator M. tuberculosis ESAT-6 is a peptide pool covering the complete sequence of the ESAT-6 protein (UniProtKB Acc. no P9WNK7).
In vitro stimulation of antigen-specific T cells with PepTivator Peptide Pools causes the secretion of effector cytokines and the up-regulation of activation markers, which then allow the detection and isolation of antigen-specific T cells.