Miltenyi, 130-132-979, REAlease® CD279 MicroBead Kit, human

Background information
The REAlease CD279 MicroBead Kit has been developed for the isolation of CD279+ cells from PBMCs. The REAlease CD279 MicroBead Kit is an indirect magnetic labelling system that allows to obtain cells free of MicroBeads and REAlease Biotin Complex.

Detailed separation procedure
CD279, also known as PD-1 (programmed cell death 1), is expressed on T cells, B cells, NK cells, activated myeloid cells, and dendritic cells are labeled with REAlease CD279-Biotin (REAlease Biotin Complex). Subsequently, REAlease Anti-Biotin MicroBeads (CD279, human) bind to the REAlease Biotin Complex. Then, the cell suspension is loaded onto a MACS Column, which is placed in the magnetic field of a MACS Separator. The magnetically labeled cells are retained within the column. The unlabeled non-target cells flow through; this cell fraction is thus depleted of CD279+ cells.

Downstream applications
Isolation of CD279+ label free cells. The selected CD279+ cells can be sorted according to a second marker of interest, for example, CD8, CD4, CD137 (4-1BB), CD223 (LAG-3), or CD366 (TIM-3).

Columns
MS or LS Columns.

Overview
The REAlease® CD279 MicroBead Kit was developed for rapid positive selection of CD279+ cells from PBMCs. The purified target cells are bead-free, practical for consecutive magnetic isolations, and can be label-free so that epitopes are available for any downstream application.