Miltenyi, 130-132-990, StemMACS™ iPSC mRNA Reprogramming Kit, human

Background information
With the StemMACS iPSC mRNA Reprogramming Kit, human iPSCs can be generated within 14 days under feeder-free conditions without the need for a conditioned medium or the use of B18R protein. The used mRNA-based approach in contrast to traditional viral- or DNA-based reprogramming methods eliminates the risk for genomic integration and the safety concerns connected with using viral-based vectors. There is no need for subsequent screening and laborious experiments to confirm the elimination of the exogenous factors. The StemMACS mRNA Reprogramming Cocktail, human contains transcripts of the following genes: POU5F1 (OCT3/4 ), SOX2, KLF4, MYC, NANOG, LIN28A, and SOCS1. The StemMACS iPSC mRNA Reprogramming Kit, human is an all-inclusive reagent set, containing the mRNA cocktails, transfection reagents, and reprogramming medium needed for the whole reprogramming process. The kit supports reprogramming of nine wells in 6-well plate format.

Applications
mRNA-based reprogramming of human fibroblasts into iPSC

Overview
The StemMACS iPSC mRNA Reprogramming Kit, human has been designed for the rapid and highly efficient reprogramming of human fibroblasts into induced pluripotent stem cells (iPSCs).