Description
MACS® UltraMounts
With the MACS UltraMounts workflows, you can harness the power of the UltraMicroscope Choros like never before.
All MACS UltraMount holders have open sides to enable optimal light sheet illumination of your samples. Some of the designs enable you to perform tissue labeling and clearing while your samples are loaded into the holders, so you can batch both tissue clearing and imaging into one easy workflow. You can find a sample prep protocol for staining and clearing of samples embedded in the MACS UltraMount 48 in the Resources section on this page.
The MACS UltraMount sample arm for UltraMicroscope Choros is compatible with all MACS UltraMount sample holder for the UltraMicroscope Blaze, for imaging of up to 48 organoids or 12 xenografts, for example.
Additionally, The MACS UltraMount 36 is specifically designed for UltraMicroscope Choros, and it features 36 wells sized 7 mm × 7 mm × 5 mm (w × d × h) for high-throughput imaging of embedded specimens.
Each UltraMicroscope Choros™ comes with standard MACS® UltraMount Template Masks, enabling you to quickly and reliably set up batch modes to image your samples, set acquisition parameters, and then let the automation of the UltraMicroscope Choros™ take it from there. You will find an application protocol guiding you through the batch measurement setup process in the Resources section on this page.
Using MACS UltraMounts in combination with the MACS® Clearing Kit or MACS® Deep Clearing Kit and the Miltenyi 3D-IF validated antibodies, you have end-to-end tested protocols for fast and reliable tissue preparation prior to imaging.
Available protocols, as well as editable templates, are available in the Resources section on this page.
Chambers and sample holders for UltraMicroscope Choros
The cuvette of the UltraMicroscope Choros (7.1 cm × 25.0 cm × 9.0 cm, w × d × h) allows convenient imaging of samples as large as a whole mouse or an entire human organ, for a total imaging volume of 69 cm3.
The whole mouse sample holder designed for UltraMicroscope Choros™ (10 mm × 90 mm × 14 mm, w × d × h), enables effortless mounting of samples as big as a whole cleared mouse on the Large sample stage arm for the UltraMicroscope Choros.
To further increase the versatility of your Ultramicroscope, a selection of application-oriented 3D printed sample holders is also available. These holders are characterized by a clamping mechanism that ensures a tight adhesion to the sample arm, hence ruling out sample movement artefacts during image acquisition. The standard sample stage arm allows for all UltraMicroscope Blaze sample holders to be used on the UltraMicroscope Choros systems as well.
Overview
The boundaries of 3D imaging can be expanded with a wide range of accessories and upgrades for the UltraMicroscope Choros™ System. Whole adult mice can be imaged with the dedicated sample holder, and in addition, sample throughput can be enhanced significantly by the MACS® UltraMount Sample Holders and related workflows. The LightSpeed Mode for UltraMicroscope systems provides the fastest 3D imaging speeds available. Furthermore, each system can be configured to match specific experimental requirements through a selection of application-oriented sample holders. Application protocols are available in the Resources section.
