Miltenyi, 130-133-881, MSLN CAR Detection Reagent, human, Biotin

Overview
The MSLN CAR Detection Reagent, anti-human, Biotin, REAfinity™ has been developed for the detection of transduced T cells that are engineered to express MSLN-specific chimeric antigen receptors (CAR) on the cell surface, which recognize human MSLN antigen.
The MSLN CAR Detection Reagent is an antigen based detection reagent conjugated to biotin. It contains a recombinantly expressed fusion protein consisting of the human MSLN extracellular domains and a specifically mutated human IgG1 Fc region.
The engineered MSLN CAR T cells can be detected via the recognition of the MSLN protein, and identified by flow cytometry via anti-biotin fluorochromes. The mutated human IgG1 Fc region of the MSLN CAR Detection Reagent abolishes their binding to Fcγ receptors. This allows for background-free analysis and eliminates the need for additional blocking steps, such as using a FcR blocking reagent.