Description
Background information
FFPE Tissue Dissociation Kit for RNA Profiling
The FFPE Tissue Dissociation Kit for RNA Profiling is used for the preparation of single-nuclei suspensions from FFPE tissue for downstream RNA sequencing analysis.
The FFPE Tissue Dissociation Kit for RNA Profiling efficiently dissociates formalin-fixed paraffin-embedded (FFPE) tissue samples while preserving nuclear integrity and minimizing RNA degradation, leading to a robust outcome and reliable interpretation of nuclear RNA content. The optimized enzyme mixture significantly reduces mitochondrial RNA contamination, further enhancing data quality.
Single-nucleus suspensions can be easily obtained from FFPE tissue sections using the FFPE Tissue Dissociation Kit for RNA Profiling on the gentleMACS Octo Dissociator with Heaters and gentleMACS C Tubes. The reagents of the kit allow efficient extraction of nuclei while removing unwanted noise from e.g. mitochondrial genes, increasing the reliability and reproducibility of genomic analysis. The isolated nuclei from different FFPE tumor samples have been validated for 10x Genomics’ “Chromium Fixed RNA Profiling” analysis.
FFPE Tissue Dissociation Kit
The FFPE Tissue Dissociation Kit is used to dissociate cells for further isolation of tumor cells using e.g. Anti-Cytokeratin MicroBeads, human (# 130-123-094) to increase the specificity of downstream bulk DNA sequencing results.
FFPE tissue sections from various tissues and species, for example, human tumor, skin, or lymph node samples, can be dissociated into single-cell suspensions by combining mechanical dissociation with enzymatic degradation of the extracellular matrix. The FFPE tissue sections are deparaffinized and rehydrated in this process. Afterwards, samples are dissociated using the kit components and a gentleMACS™ Dissociator with Heaters and gentleMACS C Tubes. Larger particles are removed subsequently using MACS SmartStrainers.
This kit is specifically designed to achieve high single-cell yields while obtaining cytokeratin and vimentin epitopes relevant for carcinoma cell analysis. Single-cell suspensions are indicated for subsequent isolation of carcinoma cells, flow cytometric analysis, or molecular analysis such as bulk DNA Seq.
Overview
The FFPE Tissue Dissociation Kit for RNA Profiling is specifically developed for the efficient and rapid isolation of nuclei from FFPE tissue for subsequent analysis using RNA sequencing, e.g. the 10x Genomics’ “Chromium Fixed RNA Profiling” assay.
The FFPE Tissue Dissociation Kit enables gentle, rapid, and effective generation of single-cell suspensions from human FFPE tissue sections. Single-cell suspensions can subsequently be used for isolation of carcinoma cells, flow cytometry, or molecular analysis such as bulk DNA sequencing.
