Miltenyi, 130-135-455, StraightFrom® Whole Blood REAlease® Anti-TCRγ/δ MicroBead Kit, human

Detailed separation procedure
TCRγ/δ+ T cells are labeled with StraightFrom Whole Blood REAlease Anti-TCRγ/δ-Biotin, human (REAlease Biotin Complex). Subsequently, StraightFrom Whole Blood REAlease Anti-Biotin MicroBeads (Anti-TCRγ/δ, human) bind to REAlease Biotin Complex. Then, the cell suspension is loaded onto a MACS Column, which is placed in the magnetic field of a MACS Separator. The magnetically labeled cells are retained within the column. The unlabeled non-target cells flow through; this cell fraction is thus depleted of TCRγ/δ+ T cells. Using the REAlease Bead Release Reagent, which removes the MicroBeads from the cell, target cells are eluted within the magnetic field. Finally, during the subsequent incubation with the REAlease Release Reagent, the REAlease Biotin Complex monomerizes and dissociates from the cell surface leaving the cells free of all labels.

Applications
Isolation of TCRγ/δ+ T cells from whole blood. The purified TCRγ/δ+ T cells are well suited for further flow cytometric, functional or molecular analysis.

Columns
Whole Blood columns, Multi-24 Whole Blood Column Block

Overview
The StraightFrom Whole Blood REAlease Anti-TCRγ/δ MicroBead Kit, human was developed for rapid positive selection of TCRγ/δ+ T cells directly from whole blood. No sample preparation is required, including density gradient centrifugation or erythrocyte lysis. The purified target cells are bead-free, practical for consecutive magnetic isolations, and can be label-free so that epitopes are available for any downstream application.