Description
Background information
This pre-defined panel of liquid MACS antibodies has been developed for MICS on FFPE-fixed human samples. The panel kit will be provided with a set of pre-tested staining and imaging parameters for the MACSima System. This panel can serve as a starting point, to quickly gain an overview over a tissue and then adopted to suit a specific sample.
The list of markers including what structures they help can help identify is listed below:
Lymphoid cells
- CD45 - Immune cells
- CD3 - T cells
- CD56 - NK cells
- CD20 - B cells
Myeloid cells
- CD11c - Dendritic cells
- CD68 - Macrophages
- CD163 - M2-like macrophages
- CD66b - Neutrophils
T-cells
- CD8a - Cytotoxic T cells
- CD4 - Helper T cells
- FoxP3 - Regulatory T cells
- CD279 (PD1) - Immunosuppression
Tissue features
- Pan-Cytokeratin - Epithelium/tumor
- CD326 (EpCAM) - Epithelium/tumor
- CD31 - Endothelia
- Actin (Smooth Muscle) - Blood vessels
- Podoplanin - Lymphatic vessels
- Ki-67 - Proliferation
- Cleaved PARP1 - Apoptosis
- CD274 (PD-L1) - Immunosuppression
Overview
The REAplex IO Core human, FFPE panel is a pre-defined panel of 20 liquid antibody conjugates. It is designed as a standardized basis for spatial mapping of formalin-fixed-paraffin-embedded (FFPE) human tumor tissue samples on the MACSima platform. The markers it contains stain key tissue structures to distinguish healthy from tumor tissue, and allow identification of tumor origin and malignancy.
Key immune cell markers allow identification of tumor-invading immune cells and give information about their activity.
