Miltenyi, 130-136-454, MACSmatrix Laminin 511

Background information
Laminins are proteins that are important for correct cell functionality. They are essential for the culture of stem cells and tissue-specific cells by controlling cell behaviour and cell fate. The type of laminin presents changes during the differentiation process of cells. In cell culture, stem cells grow as monolayers on laminin enabling contact to both the laminin matrix and the cell culture medium forming a homogenous population of cells. The laminin 511 isoform is instrumental to the growth and maintenance of human pluripotent stem cells (hPSCs) through its binding to cell receptors α6β1 integrin. The E8 fragment of laminin 511 has the highest binding activity with integrin α6β1. MACSmatrix Laminin 511 enables extended culture of human pluripotent stem cells keeping the expression of pluripotency markers, preserving genomic integrity and keeping the differentiation capability of cells. MACSmatrix Laminin 511 is Animal Component Free (ACF).

Each lot of MACSmatrix Laminin 511 is tested for
- Biological activity
- Concentration
- Microbial contamination
- Endotoxins
- Mycoplasma
- Purity
Download the lot-specific CoA from our Support section

Applications
- Culture of hPSCs
- Culture of iPS-derived cardiomyocytes
- Reprogramming of fibroblasts into iPSCs

Overview
MACSmatrix Laminin 511 is a highly pure, human recombinant protein (E8 fragment) produced in CHO cells.