Description
Background information
HHV-2, also known as herpes simplex virus (HSV-2), belongs to the family of Herpesviridae which are widespread among humans. Once infected, the virus persists for life. Thereby the envelope glycoprotein, which shows 81.9 % sequence homology between HHV-1 and HHV-2 subtype, binds to host cell receptors and promotes the virus entry into the cell.
The Herpesviridae are not only known to cause various diseases like Herpes labialis or Herpes genitalis, but also life threatening complications in immunocompromised individuals (e.g., HHV induced encephalitis).
The PepTivator Peptide Pools have been specially developed for efficient in vitro stimulation of antigen-specific CD4+ and CD8+ T cells, as peptides of 15 amino acid length with 11 amino acid overlap represent the optimized solution for stimulating both CD4+ and CD8+ T cells in various applications.
Stimulation of T cells with PepTivator Peptide Pools causes the secretion of effector cytokines and upregulation of activation markers, which then allows the detection or isolation of antigen-specific T cells.
Quantitative, phenotypical, or functional analysis of antigen-specific T cell immunity can provide important information on the natural course of immune responses.
Overview
PepTivator HHV-2 Envelope Glycoprotein D is a peptide pool that consists mainly of 15-mer sequences with 11 amino acids (aa) overlap, covering the complete sequence of the human herpes virus type 2 (HHV-2) envelope glycoprotein D (UniProtKB Acc. no. P03172).
In vitro stimulation of antigen-specific T cells with PepTivator Peptide Pools causes the secretion of effector cytokines and the up-regulation of activation markers, which then allow the detection and isolation of antigen-specific T cells.
