Miltenyi, 130-136-717, StraightFrom® Whole Blood REAlease® CD4 MicroBead Kit, human

CD4+ cells were isolated from whole blood using the StraightFrom Whole Blood REAlease CD4 MicroBead Kit, human and Whole Blood Columns. Cells were fluorescently stained with the REAfinity Antibody CD4-PE and analyzed by flow cytometry using the MACSQuant® Analyzer X. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. The CD4+ T cell content of the isolated fraction is typically 99% ± 1 (mean ± SD).

Downstream applications
StraightFrom Whole Blood REAlease CD4 MicroBead Kit allows the fast isolation of CD4+ T cells directly from blood thus minimizing hands-on time and maximizing yields of target cells. The purified CD4+ T cells are well-suited for further flow cytometric, functional, or molecular analysis. The isolated CD4+ T cell subset is label-free.

Columns
Whole Blood Columns, Multi-24 Whole Blood Column Block, autoMACS Columns

Overview
StraightFrom® Whole Blood REAlease® CD4 MicroBead Kit was developed for rapid positive selection of CD4+ cells directly from whole blood. No sample preparation is required, including density gradient centrifugation or erythrocyte lysis. The purified target cells are bead-free, practical for consecutive magnetic isolations, and can be label-free so that epitopes are available for any downstream application.