Miltenyi, 130-137-251, CAR Antibody (Whitlow/218 Linker), PE, REAfinity™

Name: CAR Antibody (Whitlow/218 Linker), PE, REAfinity™
Brand: Miltenyi
SKU: 130-137-251

Size:30 tests in 60 µL, SupT1 cells expressing a CAR construct that includes the Whitlow/218 Linker were stained with CAR Antibody (Whitlow/218 Linker), REAfinity and CD4 antibodies. Flow cytometry analysis was performed using the MACSQuant® Analyzer. Cell debris and dead cells were excluded based on scatter signals and 7-AAD fluorescence.

Catalog Number: 130-137-251

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Category

Cell Cycle Reagents

Pack Size30 tests

TagsMACS Flow Cytometry Flow cytometry reagents and kits CAR Detection Reagents

Description

Overview
The CAR Antibody (Whitlow/218 Linker), REAfinity™ detects transduced T cells expressing chimeric antigen receptors (CARs) that incorporate the Whitlow/218 linker peptide, a common structural motif in CAR designs. This enables recognition of a broad range of CAR constructs, independent of antigen specificity, by targeting the linker region.
Its mutated human IgG1 Fc region prevents Fcγ receptor binding, ensuring background-free analysis without the need for additional FcR blocking steps.

Specifications

General

Clone	REA1400
Clonality	monoclonal
Isotype control	REA Control Antibody (S), human IgG1

Ordering

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