Miltenyi, 130-139-240, MACS® qPCR LENTIcheck Kit (BaEV), human

Name: MACS® qPCR LENTIcheck Kit (BaEV), human
Brand: Miltenyi
SKU: 130-139-240

Size:for 96 reactions

Catalog Number: 130-139-240

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Category

Sequencing Reaction Kits

TagsMACS Molecular Analysis NGS and PCR VCN and RCL assays

Description

Overview
TheMACS qPCR LENTIcheck Kit (BaEV), human is a qPCR-based assay developed for the rapid assessment of replication competent lentivirus (RCL) in cell-based products transduced with lentiviral vectors provided by Lentigen and Miltenyi Biotec. The MACS qPCR LENTIcheck Kit (BaEV), human detects the lentiviral envelope gene sequences (Baboon envelope (BaEV) gene). The envelope gene (BaEV) is the most suitable target for detecting RCLs for the usage of vectors that contain this gene, since the sequence must be incorporated to generate a replication competent virus. The absence of the BaEV gene in ex vivo transduced cells according to the PCR assay can be used for lot release testing of cell-based products, in particular, when time constrains are present.
Important note: The primers included in the MACS qPCR LENTIcheck Kit (BaEV), human are designed to amplify an amplicon of the lentiviral envelope gene sequence (BaEV). These primers are intended to be used with the lentiviral vectors provided by Lentigen and Miltenyi Biotec.
For information about compatibility with other lentiviral vectors, please contact the Miltenyi Biotec Technical Support team.

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