Description
Background information
CD4 is a 55 kDa protein that is highly expressed on T helper cells in the thymus and peripheral blood. In T helper cells, CD4 functions as an accessory molecule for the recognition of MHC class II/peptide complexes by TCR heterodimers. CD4 is also expressed on monocytes and dendritic cells, albeit at lower levels than on CD4+ T helper cells. In conjunction with certain chemokine receptors, CD4 is the primary high-affinity receptor for HIV.
Downstream applications
Isolation of CD4+ T helper cells is performed in many different fields of research, such as infectious diseases, autoimmune diseases, allergy, asthma, and tumor immunology. T helper cells isolated by MACS® Technology remain viable and functional. Therefore, they can be used for further functional studies, such as proliferation assays and cytotoxicity assays, as well as for the generation of T cell lines. T helper cells isolated by MACS® Technology were also used for studies on viral infections, e.g., for in vitro infections of cells with HIV, to determine the infectivity of HIV-infected follicular dendritic cells, immune reconstitution after anti-viral therapy, and for monitoring of immune abnormalities in children of HIV-infected mothers. Isolation or depletion of CD4+ T helper cells is performed in many different fields of research, such as infectious diseases, autoimmune diseases, allergy, asthma, and tumor immunology. T helper cells isolated by MACS® Technology remain viable and functional. Therefore, they can be used for further functional studies, such as proliferation assays and cytotoxicity assays, as well as for the generation of T cell lines. T helper cells isolated by MACS® Technology were also used for studies on viral infections, e.g., for in vitro infections of cells with HIV, to determine the infectivity of HIV-infected follicular dendritic cells, immune reconstitution after anti-viral therapy, and for monitoring of immune abnormalities in children of HIV-infected mothers.
Columns
For positive selection: MS, LS, XS, or autoMACS® Columns. For depletion: LD, D, or autoMACS Columns.
Overview
CD4 MicroBeads UltraPure, human are MicroBeads conjugated to monoclonal anti human CD4 antibodies (isotype: recombinant human IgG1). They were developed for positive selection of cells expressing human CD4 antigen from peripheral blood mononuclear cells (PBMCs), leukapheresis, and cord blood. In addition, T helper cells have also been isolated from lymph nodes, thymus, spleen, skin biopsies, synovial fluids, and cell cultures. When working with anticoagulated peripheral blood or buffy coat, peripheral blood mononuclear cells (PBMCs) should be isolated first.
