Description
Background information
This pre-defined panel of liquid MACS antibodies has been developed for MICS on PFA-fixed frozen mouse brain tissue sections. This panel can serve as a starting point to quickly gain an overview over a tissue and then adopted to suit a specific sample. It provides the full workflow to dive into the spatial biology of your sample.
- Optimized set of antibody conjugates, curated to stain markers that identify key cell populations and tissue structures in your sample
- Pre-tested imaging parameters in complete instrument procedure are an ideal springboard to quickly achieve ideal settings for your specific sample
- Application protocol as a step-by-step guide on how to use the kit with the MACSima™ Platform
- The included analysis guideline walks you through the spatial data analysis with MACS® iQ View
Neural Markers
- NeuN – Mature Neurons
- MAP2 – Dendrites
- β-Tubulin 3- Immature Neurons
- Tau – Axons
- Neurofilament – Neurites
- Synapsin-1 – Synapses
- Synaptophysin – Synapses
- Tyrosine Hydroxylase – Dopaminergic synapses
- Nestin – Neural Progenitor Cells (NPCs)
Glial and Vascular Markers
- GLAST (ACSA-a) – Radial glia, neural stem cells
- S100B – Astrocytes
- GFAP – Astrocytes
- O4 – Oligodendrocytes
- Mog – Mature Oligodendrocytes
- MPB – Myelinating Oligodendrocytes
- PLP - Myelinating Oligodendrocytes
- Iba1 (AIF1) – Microglia
- CD68 – (activated) Microglia
- CD11b – (activated) Microglia
- CD105 – (activated) Blood vessels
Overview
The REAplex Neuro Core Kit is a pre-defined panel of 20 liquid antibody conjugates. It is designed as a standardized basis for spatial mapping of frozen mouse brain tissue sections on the MACSima™ platform. The included antibody conjugates stain key brain structures, allowing visualization of neurons, and identification of glial and immune cell populations.
Gain insight into the spatial complexity of neurodegenerative disease, such as Alzheimer’s or Parkinson’s disease and follow their disease progression.
