Miltenyi, 130-139-854, B7-H3 CAR Detection Reagent, human, Biotin

Overview
The B7-H3 CAR Detection Reagent, human, Biotin has been developed for the detection of transduced T cells that are engineered to express B7-H3-specific chimeric antigen receptors (CAR) on the cell surface, which recognize human B7-H3 antigen.
The B7-H3 CAR Detection Reagent is an antigen based detection reagent conjugated to biotin. It contains a recombinantly expressed fusion protein consisting of the human B7-H3 extracellular domains and a specifically mutated human IgG1 Fc region.
The engineered B7-H3 CAR T cells can be detected via the recognition of the B7-H3 protein, and identified by flow cytometry via anti-biotin fluorochromes. The mutated human IgG1 Fc region of the B7-H3 CAR Detection Reagent abolishes their binding to Fcγ receptors. This allows for background-free analysis and eliminates the need for additional blocking steps, such as using a FcR blocking reagent.