Description
The Express Mode Master Package contains three Express Mode Packages with a total of 17 Express Modes:
The CD19 CAR T Cell Express Mode Package contains 12 optimized application-specific Express Modes:
- Immune_Cell_Composition_h_02: For the determination of cell viability and composition of immune cells.
- CAR_T_Cell_Transduction_h_02: For the determination of transduction efficiency of CAR T cells.
- B_Malignant_Cells_h_01: For the determination of malignant B cells.
- B_Regulatory_Cells_h_01: For the determination of various subsets of regulatory B cells.
- B_Cell_Differentiation_h_02: For the determination of B cell differentiation.
- CAR_T_Cell_Tscm_h_01: For the determination of stem memory T cells (Tscm).
- CAR_T_Cell_Persistence_h_01: For the determination of CAR T cell persistence.
- CAR_T_Cell_Differentiation_h_01: For the analysis of differentiation markers on CAR+ and CAR– T cells.
- CAR_T_Cell_Proliferative_Ability_h_01: For the analysis of proliferative ability markers on CAR+ and CAR– T cells.
- CAR_T_Cell_Exhaustion_h_01: For the analysis of exhaustion markers on CAR+ and CAR– T cells.
- CAR_T_Cell_Activation_h_01: For the analysis of activation markers on CAR+ and CAR– T cells.
- CAR_T_Cell_Staining_Control_h_01: For optional staining controls.
The Virus-Specific T Cell CCS Express Mode Package contains three optimized application-specific Express Modes:
- RCI_CD4CD8_h_02: For the determination of IFN-y expression of target cells (analysis of pre-donor test).
- CCS_Immune_Cell_Composition_h_01: For the determination of cellular composition and target cell number.
- CCS_Purity_h_01: For the determination of the phenotype and frequency of target cells.
The TCRαβ/CD45RA Express Mode Package contains two optimized application-specific Express Modes:
- TCRαβ_CD45R_depletion_h_02: For the determination of the frequency of CD45RA+, TCRα/β+, and CD34+ cells.
- Immune_Cell_Composition_human: For the determination of cell viability and composition of immune cells.
Overview
Express Modes are a powerful add-on for the MACSQuantify™ Software, designed to simplify flow cytometry. They standardize your assays through predefined experiment settings and fully automated sample acquisition, data analysis, and reporting. Dynamic gating ensures that parameters are automatically adjusted for each data file, delivering accurate identification of cell populations. By using Express Modes, you minimize operator variability and achieve consistent, reproducible results every time.
A detailed application protocol can be found under the Resources tab.
