Miltenyi, 170-076-102, MACS® GMP Recombinant Human IL-1β

Biological activity

Background information
IL-1β is mainly secreted by monocytes and macrophages. IL-1β secretion has also been reported for a variety of other cells, including B cells, NK cells, dendritic cells, astrocytes, and microglial cells. IL-1β acts also on nonimmune system cells such as fibroblasts, vascular endothelial cells, and hepatocytes.

Applications
MACS GMP Recombinant Human IL-1β can be used for a variety of applications, including the ex vivo generation of human dendritic cells from enriched CD14+ monocyte populations.2–4

Technical specifications
- Identity/ Molecular mass: 17377 Da, determined by mass spectrometry. This corresponds to the mature form of human IL-1β (153 amino acids).
- Purity: ≥ 97 % as determined by chip electrophoresis.
- Endotoxin content: < 50 EU/mg, as determined by kinetic Limulus Amoebocyte Lysate (LAL) assay (Pharmacopoeia Europaea (Ph. Eur.)).
- Residual host cell DNA content: < 5 ng/mg, as determined by quantitative PCR specific for E. coli genomic DNA.
- Residual host cell protein content: < 0.5 µg/mg, as determined by E. coli HCP ELISA.
The product quality certificate of MACS® GMP Recombinant Human IL-1β was adapted. Learn more here

Quality statement
MACS GMP Products are manufactured and tested under a quality management system (ISO 13485) and are in compliance with relevant GMP guidelines. They are designed following the recommendations of USP <1043> on ancillary materials.

Disclaimer
MACS GMP Products are for ex vivo cell processing only, and are not intended for human in vivo applications. For regulatory status in the USA, please contact your local representative.

Overview
Interleukin-1β (IL-1β) is a pro-inflammatory cytokine that mediates inflammatory responses and displays a wide variety of biological activities on many different cell types, including T cells, B cells, and monocytes.
MACS GMP Recombinant Human IL-1β is designed for ex vivo cell culture processing. No animal- or human-derived materials were used for the manufacture of this product, unless otherwise stated in the respective Certificate of Origin. The product is lyophilized without carrier protein or preservatives.