Description
Background information
Human adenoviruses (AdV) are ubiquitous lytic DNA viruses, consisting of multiple serotypes and subspecies. AdV infections are associated with significant morbidity and mortality after hematopoetic stem cell transplantations.
The hexon protein is one of the three major proteins of the viral capsid and an immunodominant target antigen for CD4+ and CD8+ T cells that is conserved between the serotypes and subspecies.
Applications
Antigen-specific T cells play a critical role in the regulation of immune responses and the elimination of virus-infected or malignant cells in the human body. Adoptive transfer of clinical-grade hexon-specific CD4+ and CD8+ T cells is a valuable tool for the treatment of AdV infections after hematopoietic stem cell transplantation (SCT).1
The in vitro stimulation of hexon-specific CD4+ and CD8+ T cells with MACS GMP PepTivator AdV5 Hexon causes the production of the effector cytokine IFN-gamma. The secretion of IFN-gamma then permits the enrichment of hexon-specific effector/memory T cells with the use of the CliniMACS Cytokine Capture System (IFN-gamma).2-4
Quality statement
MACS GMP Products are manufactured and tested under a quality management system (ISO 13485) and are in compliance with relevant GMP guidelines. They are designed following the recommendations of USP <1043> on ancillary materials.
Disclaimer
MACS GMP Products are for ex vivo cell processing only, and are not intended for human in vivo applications. For regulatory status in the USA, please contact your local representative.
Overview
The MACS GMP PepTivator AdV5 Hexon is a peptide pool that consists mainly of 15-mer peptides with eleven amino-acid overlap. It has been developed for efficient in vitro stimulation and subsequent isolation AdV5 hexon-specific CD4+ and CD8+ T cells.
