Miltenyi, 170-076-111, MACS® GMP Recombinant Human IL-7

Biological activity

Background information
The primary sources of IL-7 are non-hematopoietic stromal cells in bone marrow, thymus, and lymphoid organs and tissues. IL-7 is a member of the type I cytokine family. It is related to IL-2 and signals through a heterodimeric receptor composed of the common cytokine signaling γ-chain and IL-7 receptor α-chain.

Applications
MACS GMP Recombinant Human IL-7 can be used for a variety of applications, including the ex vivo generation of antigen-specific cytotoxic T lymphocytes.2,3

Technical specifications
- Identity/ Molecular mass: 17.382 Da, determined by mass spectrometry. This corresponds to the mature form of human IL-7 (152 amino acids).
- Purity: ≥ 95 % as determined by chip electrophoresis.
- Endotoxin content: < 100 EU/mg, as determined by kinetic Limulus Amoebocyte Lysate (LAL) assay (Pharmacopoeia Europaea (Ph. Eur.)).
- Residual host cell DNA content: < 20 ng/mg, as determined by quantitative PCR specific for E. coli genomic DNA.
- Residual host cell protein content: < 5 µg/mg, as determined by E. coli HCP ELISA.

The product quality certificate of MACS® GMP Recombinant Human IL-7 was adapted. Learn more here

Quality statement
MACS GMP Products are manufactured and tested under a quality management system (ISO 13485) and are in compliance with relevant GMP guidelines. They are designed following the recommendations of USP <1043> on ancillary materials.

Disclaimer
MACS GMP Products are for ex vivo cell processing only, and are not intended for human in vivo applications. For regulatory status in the USA, please contact your local representative.

Overview
Interleukin 7 (IL-7) is a pleiotropic cytokine with central roles in modulating T cell development and peripheral T cell homeostasis. IL-7 can act both as a T cell growth factor as well as a critical anti-apoptotic survival factor for naive and memory T cells.
MACS GMP Recombinant Human IL-7 is designed for ex vivo cell culture processing. No animal- or human-derived materials were used for the manufacture of this product, unless otherwise stated in the respective Certificate of Origin. The product is lyophilized without carrier protein or preservatives.