Description
Biological activity
Background information
IL-21 is a four α-helix bundle cytokine and closely related to IL-2, IL-7, and IL-15. IL-21 expression is restricted to activated CD4+ T helper cells and NKT cells. The functional receptor for IL-21, composed of the IL-21 receptor- and the common γ-chain, is expressed on various hematopoietic cells including T, B, NK and dendritic cells.
Applications
MACS GMP Recombinant Human IL-21 can be used for a variety of applications, including the ex vivo generation of antigen-specific cytotoxic T cells1,2 or the ex vivo stimulation of NK cells3.
Technical specifications
- Identity/Molecular mass: 15460 Da, determined by mass spectrometry. This corresponds to the mature form of human IL-21 (133 amino acids).
- Purity: ≥ 95 % as determined by chip electrophoresis.
- Endotoxin content: < 100 EU/mg, as determined by kinetic Limulus Amoebocyte Lysate (LAL) assay (Pharmacopoeia Europaea (Ph. Eur.)).
- Residual host cell DNA content: < 20 ng/mg, as determined by quantitative PCR specific for E. coli genomic DNA.
- Residual host cell protein content: < 5 µg/mg, as determined by E. coli HCP ELISA.
The product quality certificate of MACS® GMP Recombinant Human IL-21 was adapted. Learn more here
Quality statement
MACS GMP Products are manufactured and tested under a quality management system (ISO 13485) and are in compliance with relevant GMP guidelines. They are designed following the recommendations of USP <1043> on ancillary materials.
Disclaimer
MACS GMP Products are for ex vivo cell processing only, and are not intended for human in vivo applications. For regulatory status in the USA, please contact your local representative.
Overview
Interleukin 21 (IL-21) has pleiotropic effects on both cellular and humoral immune responses, such as stimulation of lymphocyte proliferation, promotion of CD8+ T cell and NK cell cytotoxicity, and differentiation of B cells into plasma cells.
MACS GMP Recombinant Human IL-21 is designed for ex vivo cell culture processing. No animal- or human-derived materials were used for manufacture of this product. The product is lyophilized without carrier protein or preservatives.
