Description
Biological activity
Background information
Flt3-Ligand belongs to a small family of α-helical cytokines and is expressed by T lymphocytes and bone marrow stromal fibroblasts as a membrane-bound and a soluble isoform. Both isoforms signal through the tyrosine kinase receptor Flt3/Flk-2, which is restricted to cells of hematopoietic origin.
Applications
MACS GMP Recombinant Human Flt3-Ligand can be used for a variety of applications, including the expansion2,3 and the viral transduction4,5 of hematopoietic stem and progenitor cells derived from bone marrow, peripheral blood cells, and cord blood.
Technical specifications
- Identity/ Molecular mass: 17934 Da, determined by mass spectrometry. This corresponds to the mature form of human Flt3-Ligand (158 amino acids).
- Purity: ≥ 97 % as determined by chip electrophoresis.
- Endotoxin content: < 25 EU/mg, as determined by kinetic Limulus Amoebocyte Lysate (LAL) assay (Pharmacopoeia Europaea (Ph. Eur.)).
- Residual host cell DNA content: < 5 ng/mg, as determined by quantitative PCR specific for E. coli genomic DNA.
- Residual host cell protein content: < 5 µg/mg, as determined by E. coli HCP ELISA.
The product quality certificate of our MACS® GMP Recombinant Human GM-CSF has been adapted. Learn more here
Quality statement
MACS GMP Products are manufactured and tested under a quality management system (ISO 13485) and are in compliance with relevant GMP guidelines. They are designed following the recommendations of USP <1043> on ancillary materials.
Disclaimer
MACS GMP Products are for ex vivo cell processing only, and are not intended for human in vivo applications. For regulatory status in the USA, please contact your local representative.
Overview
Fms-related tyrosine kinase 3 ligand (Flt3-Ligand) is a growth factor that exerts pleiotropic and potent effects on the development of hematopoietic stem cells (HSCs) and the immune system. In synergy with other growth factors Flt3-Ligand promotes proliferation and development of HSCs, myeloid and lymphoid progenitor cells, dendritic cells, and natural killer cells.
MACS GMP Recombinant Human Flt3-Ligand is designed for ex vivo cell culture processing. No animal- or human-derived materials were used for the manufacture of this product, unless otherwise stated in the respective Certificate of Origin. The product is lyophilized without carrier protein or preservatives.
